Scaffolds |
Accurate tissue recapitulation |
Expensive, variability in polymer composition |
[14,17,18] |
Agitation-based methods |
Easy to perform, inexpensive, appropriate for multicellular spheroid generation |
Variability in spheroid size, extracellular matrix not addable, inappropriate for migration assays |
[19,20,21,22] |
Suspension cell cultures |
Easy to perform, inexpensive, appropriate for multicellular spheroid generation |
Variability in spheroid size, extracellular matrix not addable, inappropriate for migration assays |
[19,20,21,22] |
Hanging drop |
Inexpensive, spheroid size uniformity |
Difficult to perform, extracellular matrix not addable, inappropriate for migration assays |
[19,20,21,22] |
Organ-on-a-chip |
Rapid spheroid formation, spheroid size uniformity, constant perfusion and distribution of oxygen and nutrients |
Expensive, difficult to perform, specialized equipment and expertise |
[23,24] |