Table 5.
Minimal inhibition concentration (MIC), minimal bactericidal concentration (MBC), and MBC/MIC ratio showing quantitative antibacterial activity of two EOs (C. carvi and C. sativum) and their mixture against human pathogenic bacteria compared to standard antibiotics (gentamycin and tetracycline).
Microorganisms Tested | C. carvi EO | C. sativum EO | Mixture (v/v) EOs | Antibiotics | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
MIC mg/mL |
MBC mg/mL |
MBC/MIC (Interpretation) |
MIC mg/mL |
MBC mg/mL |
MBC/MIC (Interpretation) |
MIC mg/mL |
MBC mg/mL |
MBC/MIC (Interpretation) |
MIC mg/mL |
MBC mg/mL |
MBC/MIC | |
S. epidermidis CIP 106510 | 0.117 | 0.469 | 4 (Bactericidal) | 0.469 | 1.875 | 4 (Bactericidal) | 0.029 | 0.117 | 4 (Bactericidal) | 0.009 | 0.039 | 4 |
S. aureus ATCC 25923 | 0.117 | 0.469 | 4 (Bactericidal) | 0.234 | 0.938 | 4 (Bactericidal) | 0.059 | 0.234 | 4 (Bactericidal) | 0.004 | 0.019 | 4 |
M. luteus NCIMB 8166 | 0.059 | 0.234 | 4 (Bactericidal) | 0.234 | 0.938 | 4 (Bactericidal) | 0.015 | 0.059 | 4 (Bactericidal) | 0.004 | 0.019 | 4 |
E. feacalis ATCC 29212 | 0.059 | 0.469 | 8 (Bacteriostatic) | 0.469 | 0.938 | 2 (Bactericidal) | 0.117 | 0.234 | 2 (Bactericidal) | 0.004 | 0.019 | 4 |
B. cereus ATCC 11778 | 0.117 | 0.469 | 4 (Bactericidal) | 0.938 | 1.875 | 2 (Bactericidal) | 0.059 | 0.117 | 2 (Bactericidal) | 0.004 | 0.039 | 8 |
B. cereus ATCC 14579 | 0.117 | 0.469 | 4 (Bactericidal) | 0.938 | 1.875 | 2 (Bactericidal) | 0.059 | 0.234 | 4 (Bactericidal) | 0.004 | 0.039 | 4 |
E. coli ATCC 35218 | 0.469 | 1.875 | 4 (Bactericidal) | 0.938 | 3.750 | 4 (Bactericidal) | 0.117 | 0.469 | 4 (Bactericidal) | 0.009 | 0.039 | 4 |
L. monocytogenes ATCC19115 | 0.469 | 1.875 | 4 (Bactericidal) | 0.938 | 1.875 | 2 (Bactericidal) | 0.059 | 0.234 | 4 (Bactericidal) | 0.019 | 0.078 | 4 |
P. aeruginosa ATCC 27853 | 1.875 | 3.750 | 2 (Bactericidal) | 1.875 | 7.500 | 4 (Bactericidal) | 0.469 | 0.938 | 2 (Bactericidal) | 0.019 | 0.15 | 4 |
S. typhimurium LT2 DT104 | 0.234 | 0.938 | 4 (Bactericidal) | 0.938 | 1.875 | 2 (Bactericidal) | 0.059 | 0.117 | 2 (Bactericidal) | 0.019 | 0.039 | 2 |
V. parahaemolyticus ATCC17802 | 0.938 | 3.750 | 4 (Bactericidal) | 0.234 | 1.875 | 8 (Bacteriostatic) | 0.117 | 0.234 | 2 (Bactericidal) | 0.039 | 0.078 | 2 |
V. alginolyticus ATCC 33787 | 0.234 | 1.875 | 8 (Bacteriostatic) | 1.875 | 7.500 | 4 (Bactericidal) | 0.059 | 0.469 | 8 (Bacteriostatic) | 0.019 | 0.078 | 4 |
V. proteolyticus ATCC15338 | 0.469 | 1.875 | 4 (Bactericidal) | 1.875 | 3.750 | 2 (Bactericidal) | 0.117 | 0.469 | 4 (Bactericidal) | 0.009 | 0.039 | 4 |
V. furnisii ATCC 35016 | 0.469 | 3.750 | 8 (Bacteriostatic) | 1.875 | 7.500 | 4 (Bactericidal) | 0.117 | 0.469 | 4 (Bactericidal) | 0.009 | 0.039 | 4 |
V. mimicus ATCC 33653 | 0.938 | 3.750 | 4 (Bactericidal) | 1.875 | 7.500 | 4 (Bactericidal) | 0.234 | 0.938 | 4 (Bactericidal) | 0.039 | 0.078 | 2 |
V. natrigens ATCC 14048 | 1.875 | 3.750 | 2 (Bactericidal) | 1.875 | 3.750 | 2 (Bactericidal) | 0.469 | 0.938 | 2 (Bactericidal) | 0.019 | 0.039 | 2 |
V. carhiaccae ATCC 35084 | 0.938 | 1.875 | 2 (Bactericidal) | 1.875 | 7.500 | 4 (Bactericidal) | 0.117 | 0.234 | 2 (Bactericidal) | 0.039 | 0.078 | 2 |
V. fluvialis ATCC 33809 | 0.469 | 1.875 | 4 (Bactericidal) | 0.469 | 3.75 | 8 (Bacteriostatic) | 0.117 | 0.469 | 4 (Bactericidal) | 0.019 | 0.039 | 2 |