Table 1.

Impact of different SUV preparation techniques on their size and their ability to make GUVs. SUV size was characterised by dynamic light scattering (column DLS). The width of the peak is reported as error, and the reported PDI is in parenthesis. * indicates presence of a much smaller second peak corresponding to a larger object size, indicating presence of aggregates. GUVs formed using each of these category of SUVs was characterised in terms of size using bright-field microscopy (column BF). Diameter reports averages from experiments using the same batch of SUVs as DLS (between 10 and 20 GUVs were measured for each case). Average diameter reports averages from 3 different days (at least 10 GUVs each). The standard deviation is reported as error.

Technique	DLS (SUVs)	BF (GUVs)	BF (GUVs)
	Size ± Width in nm	Diameter	Average Diameter
	(PDI)	± s.d (m)	± s.d (m)
Tip Sonication	107 ± 73 (0.352) *	17 ± 7	16 ± 7
Extrusion	85 ± 44 (0.215)	14 ± 3	14 ± 4
Bath Sonication	128 ± 86 (0.334) *	7 ± 3	10 ± 4