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. 2021 Jun 14;13(12):2973. doi: 10.3390/cancers13122973

Figure 2.

Figure 2

The CTKD domain of RSK3 binds to the N-terminus of IκBα. (A) Schematics of full-length and deletion mutants (DMs) of IκBα. (B) Full-length Myc/His-RSK3 and WT pAC-GFP-IκBα WT or DM#1–4 were co-transfected into HEK293T cells. RSK3 was immunoprecipitated, and co-immunoprecipitated IκBα was identified by Western blotting using an anti-GFP antibody. Representative results from two experiments are shown. (C) Schematics of full-length and DMs of RSK3. (D) Full-length WT Myc/His-IκBα and pAC-GFP-RSK3 WT or DM1–3 were co-transfected into HEK293T cells. RSK3 was immunoprecipitated, and co-immunoprecipitated IκBα was detected by Western blotting with an anti-IκBα antibody. Representative results from three experiments are shown. (** p < 0.01 and * p < 0.05 vs. WT RSK3).