Figure 1.
Establishment of the RT-QuIC assay threshold for post-mortem brain homogenates: (A) The results of the analysis of the control brain homogenate samples (dilution 5 × 10−6) of patients with other neurological diseases (OND, n = 28). The red line represents the arithmetic mean of ThT fluorescence (expressed as arbitrary units AU) at each time point, along with the standard deviation (black bars). The threshold was calculated as the arithmetic mean of maximal ThT fluorescence + 5 SD; (B) Analysis of control brain homogenate samples (dilution 5 × 10−6) of corneal donors (CD, n = 25); (C) Typical results of the second-generation RT-QuIC assay with serial dilutions of control sCJD brain homogenate (sCJD MM1). The assay gave a positive result for the brain homogenate diluted by 5 × 10−9.