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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: FASEB J. 2021 Jul;35(7):e21733. doi: 10.1096/fj.202100405R

Figure 4: Ratio of of Scx-lin+/Scx-lin- cells in culture depends on isolation method.

Figure 4:

Scx;Ai9 mice were injected with TMX to label Scx-lin+ cells (A). Representative images showing intact FDL tendon (Masson’s Trichrome; left) and Ai9 labeling (immunofluorescence; right) of tendon cells in the same area using the described TMX regimen. Scale bars = 50 μm. Quantification (B) of Scx-lin+ and Scx-lin- tendon cells in intact FDL tendons. n=4. Representative images (C,D; top) and quantification (bottom) of Scx-lin+ (red) and Scx-lin- (gray) tendon cells isolated by digest (C) or explant culture (D). Passages are indicated by vertical gray dotted lines. Scale bars = 100 μm. n=3. Mouse model schematic was created using www.biorender.com.