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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: FASEB J. 2021 Jul;35(7):e21733. doi: 10.1096/fj.202100405R

Figure 9: Tendon cell populations present in in vitro cultures are highly dependent on isolation method and the tendon of origin.

Figure 9:

Representative images of Scx-lin+ (red) and S100a4-lin+ (blue) cells isolated from murine Achilles, flexor carpi ulnaris (FCU), and tail tendons by digest (A) or explant (B) culture. Quantification of Scx-lin+ (red) and S100a4-lin+ (blue) cells by digest (striped bars) or explant culture (solid bars) at days 4 and 16 post-isolation from Achilles (C), FCU (D), and tail (E) tendons. *p<0.05 between isolation methods at each timepoint. n=3 mice for each tendon and lineage trace. Scale bar = 100 μm.