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The HSP90 inhibitor, AUY-922, prevents the HCl-induced AKT phosphorylation. HLMVEC grown in 100 mm cultured dishes, were pre-treated for 24 h with 2 µM AUY-922 and then exposed to 0.01 N HCl. One hour later, cells were lysed, and samples were prepared for protein analysis by Western Blotting. Means ± SEM; n = 3; **: p <0.01; ***: p < 0.001 with one-way ANOVA and Tukey’s.
