Skip to main content
. 2021 Jun 13;13(12):2965. doi: 10.3390/cancers13122965

Figure 5.

Figure 5

Glyoxalase 1 (Glo1) is involved in mPCa immunosurveillance escape. DU145 and PC3 cells transiently transfected with Glo1 small interfering RNA (siGlo1) or control non-targeting small interfering RNA (siCtr) were co-cultured with CD8+ cytotoxic T cells and (a) CD8+ apoptosis was evaluated by active caspase-3 expression, with a specific ELISA kit, (b) CD8+ cell number was measured by cell counting, (c,d) TNF-α and (e,f) IL-2 secretion by CD8+ cells in the culture medium was detected by specific ELISA kit. The histograms indicate mean ± SD of three different cultures, and each was tested in duplicate. (−) untreated and (+) treated cells. * p < 0.05, ** p < 0.01; *** p < 0.001.