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Schematic illustration of RT-PCR methodology and the detection of a positive sample in clinical practice, using a specific threshold and Ct value. The SARS-CoV-2 viral load is inversely proportional to the Ct values, and a lower Ct value corresponds to a high viral load, indicating a higher level of infectiousness. The figure was designed by Biorender.com program (https://biorender.com/, accessed on 15 April 2021).
