Figure A2.

Phenotype of wild-type and hpr1 mutant plants at different growth stages and conditions. (A) The wildtype in comparison to hpr1-1 grown in normal air (390 ppm CO₂) for 5 weeks in a 12/12 h day/night cycle. Data presented in Figure 1, Figure 2 and Figure 3 were obtained from plants at this specific growth stage. Scale bar within the picture is 2 cm. (B) Phenotype of the wildtype (Col-0 and Ler) and two independent HPR1 mutant alleles (hpr1-1 and hpr1-2; [33]) grown in normal air (390 ppm CO₂) for ~7 weeks in a 12/12 h day/night cycle. The photograph illustrates that at this stage the growth retardation of hpr1 mutants is minor and onset of flowering similar between the mutants and the wildtype. Therefore, the metabolome analysis during development is comparable between both genotypes. (C) Phenotype at growth stage 5.1 [41] in short day (upper panel, after ~7 weeks) and long day (lower panel, ~4 weeks), at which leaf-material for metabolome analysis presented in Figure 6 was taken in both photoperiods. Scale bar within the picture is 2 cm.