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. 2021 Jun 15;22(12):6380. doi: 10.3390/ijms22126380

Figure 4.

Figure 4

Englerin-A induces cobalt uptake in cultured primary sensory neurons. (A) Representative images of cobalt-labelled primary sensory neurons (brown; indicated by black arrowheads) obtained from dorsal root ganglia from WT or TRPC5 −/− mice, stimulated with ascending concentrations of Englerin-A (scale bar 200 µm; magnification 10×). Capsaicin (1µM) and buffer alone were used as positive and negative controls respectively. (B) Dose-response curve of cultured primary sensory neurons from WT (black; n = 3) and KO (red, n = 3) mice to Englerin-A (C) Comparison between EC50 for Englerin A in WT DRG cultures (black) and TRPC5 KO DRG cultures (red). (D) Size-frequency distribution of cobalt-labelled DRG neurons from WT (dark grey, n = 690) and KO mice (red, n = 449) to Englerin-A (30 nM) stimulation and total capsaicin responsive neurons (light grey, n = 1453).