ML204 fails to revert EA effect on carrageenan-induced paw oedema and mechanical and thermal hyperalgesia. (A) Time-course of paw oedema measured by callipers and (B) ipsilateral/contralateral paw mass ratio 4 h following induction of paw oedema by 2% Carrageenan. Data are mean ± S.E.M, n = 5. * p < 0.05, *** p < 0.001 versus baseline, versus vehicle, Two-way ANOVA, post hoc Tukey test. (C) Myeloperoxidase (MPO) levels in paw exudates, 4 h after the induction of paw inflammation. Data are mean ± S.E.M, n = 5. * p < 0.05 versus vehicle, Unpaired t-test. (D) Thermal and (E) mechanical paw withdrawal thresholds measured at baseline and at 1 and 3 or 2 and 4 h, respectively, after intra-plantar injections of carrageenan. ** p < 0.01, *** p < 0.001 versus baseline, # p < 0.05, versus vehicle, Two-way RM ANOVA, post hoc Tukey test. Data are mean ± S.E.M, n = 5.