Figure 4.

IL1A increased cell invasiveness in TNBC cells. (A–C) after cell-seeding, TNBC cells were treated with 20 ng/mL IL1A for 24 h. (A) cell invasiveness was measured by Boyden chamber assay. The upper compartment of the insert chamber was removed using cotton swabs and the bottom filters were fixed and stained for counting invaded cells. (B,C) the levels of CCL2 (B) and IL8 (C) mRNA were measured through real-time PCR. The values are presented as the mean ± standard error of three independent experiments. * p < 0.05, ** p < 0.01 vs. Con. Con; control.