Figure 2.

Ratio and dose testing of Cas9 RNP complexed with PF14 in a DMEM/PVA-PEG buffer. (A) Ratio testing, RNP: PF14, using 10 and 50 ng Cas9 (0.61 or 2.8 nM Cas9) per well in HEK293T SL cells. n = 3 for 10 ng, n = 2 for 50 ng. There was no statistical difference (p > 0.05) of MR between 1:50 to 1:200 for the 10 ng dose as analysed by analysis of variance (ANOVA), followed by Tukey’s post-hoc test. (B) Dose titration of RNP-PF14 (MR 1:100 RNP: PF14) plotted together with RNAiMAX. Doses ranging from 1 ng (0.06 nM) to 100 ng (5.18 nM) per well of HEK293T SL cells. Linear y-axis against log¹⁰ x-axis. There was a significant difference (*, p = 0.0101) between RNAiMAX and PF14 at 2.5 ng per well, the remaining doses showed no significant difference, as analysed by two-way ANOVA followed by Bonferroni’s multiple comparison test, n = 3.