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. 2021 Jun 14;13(6):878. doi: 10.3390/pharmaceutics13060878

Figure 5.

Figure 5

Comparison of quantification techniques and cell types. (A) Shows a comparison between different quantification techniques, flow cytometry, RFLP, and NGS performed on the same samples of HEK293T SL cells treated with increasing amounts of RNP: PF14 complexes (MR 1:100 RNP to PF14). n = 1, with the flow cytometry done in triplicate, gel quant in duplicate, and NGS done in single samples. Part of the cells were analyzed by flow cytometry, the remainder of the cells were analyzed by RFLP and NGS after DNA purification and PCR amplification. (B) Displays the quantified editing of HPRT in HEK293T SL and HEK293T WT cells treated with increasing amounts of RNP: PF14 complexes (MR 1:100 RNP to PF14). The gRNA used targets the human HPRT gene. The quantification by RFLP and NGS was performed on the same samples. Gel quantification was performed in duplicate (n = 1), while NGS was done a single time per treatment (n = 1).