Figure 2. In-vitro internalization of MFN1 nanoparticles by dendritic cells.
A. Fluorescence images of JAWS II cells incubated with Alexa Fluor 488-labeled soluble M2e and NA1 or MN1 or MFN1. The fluorescence intensity of AF488 was counted using mean fluorescence intensity (MFI). Bar scale, 50 μm. B, C. Cytokines (IL-6 and TNF-α) secretion in JAWS II cells or BMDCs treated with soluble M2e and NA1 (sMN1), soluble M2e, NA1, and FliC (sMFN1), MN1, and MFN1 at a concentration of 5 μg/mL. (n = 5; **p < 0.01; ***p < 0.001; ns, p > 0.05)