Fig 2. Longitudinal evaluation of metabolic rate and RER, pre-VML surgery and at 2- and 6-weeks post-VML, in a subset of mice.

A) By 6-weeks post-VML there was decreased metabolic rate during the 24-hour and 12-hour active periods, compared to 2-weeks post-VML (p≤0.050). B) Metabolic rate was evaluated by comparing AUC values across time. When broken into 6-hour bins, significant differences were noted across time points within each bin, with mice at 6-weeks post-VML demonstrating the lowest metabolic rate in each 6-hour bin (p≤0.007). C) The RER at 6-weeks post-VML decreased over 24 hours compared to pre-VML (p = 0.013), likely due in part to a significant reduction in RER which occurred during the active period by 6-weeks post-VML (p = 0.002). D) At 2-weeks post-VML, mice exhibited a lower RER as determined by AUC compared to pre-VML, and a further significant reduction in RER at 6-weeks post-VML, indicating progressively impaired metabolic flexibility 2- and 6-weeks post-VML compared to baseline (p<0.001). In 6-hour bins, significant differences were observed across evaluation time points for each bin (p≤0.001). E) Twenty-four-hour carbohydrate oxidation 6-weeks post-VML was significantly reduced, primarily due to decreases during the active period (p = 0.002). F) Lipid oxidation over the course of 24 hours was significantly increased 6-weeeks post-VML (p<0.001) due to significantly increased oxidation during the active period. Data presented as mean±SD; analyzed by one- or two-way ANOVA; significantly different from §pre-VML; ‡VML 2wk.