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Determination of LOD with (A) phenol and (B) column-based extraction methods and confirmation of LOD using (C) phenol and (D) column-based extraction methods in 20 replicates and (E) column-based extraction method in 20 separate extractions from unique saliva samples. Black bars = N1 primer, open bars = N2 primer, gray bars = RNase P primer. Error bars = 1 standard deviation.
