Figure 4.
Stereoisomers of compound 211 show variable protective effect against STx2 toxicity. (A). Chemical structures of E- and Z- isomers of compound 211. (B,C). Cell viability assays after pre-treatment with DMSO or 10 µM indicated compounds for 24 h followed by exposure to STx2 for 18 h. LD50 with 95% confidence interval is depicted in (C). TAM, tamoxifen. N = 3 per compound. * p < 0.05 by non-linear regression for indicated differences; n.s., not significant. (D). Cell viability using varying concentrations of indicated compounds and 40 pM STx2. Other conditions were identical to (B) above. TAM, tamoxifen. N = 3 for each treatment. * p < 0.05 by two-way ANOVA with drug treatment and concentration as independent variables and Tukey–Kramer post hoc test for indicated comparisons.