Figure 9.

Tamoxifen compounds interfere with SNX1-dependent endosomal tubulation and sorting. (A). STx2B transport assay in cells transfected with GFP-SNX1 for 4 h followed by treatment with 10 µM indicated compounds for 24 h. Cells were fixed 15 min after the start of transport. Scale bars, 25 µm; inset, 5 µm. (B). Quantification of STx2B tubular extensions overlapping with SNX1 signal from (A). N = 15 cells per treatment. * p < 0.05 by one-way ANOVA and Tukey–Kramer post hoc test for indicated comparisons. (C). Quantification of SNX1 tubulation from (A). N ≥ 14 cells per treatment. * p < 0.05 by one-way ANOVA and Tukey–Kramer post hoc test for indicated differences.