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. 2021 Jun 15;10(6):1501. doi: 10.3390/cells10061501

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© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Double labeling of immuno- and conventional staining. (a): IgA and PAS in renal biopsy of IgA nephropathy. The PAS-reactive basement membrane surrounds mesangial deposition of IgA visualized after prolonged trypsin digestion (arrowheads). (b): Carcinoembryonic antigen (CEA) and alcian blue in gastric signet ring cell carcinoma. CEA expression of mucin-containing cancer cells appears weaker. (c): Insulin and Congo red in a pancreatic islet in type 2 diabetes mellitus. Orange-colored amyloid deposition is seen among the insulin-positive β-cells.