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Antigenic deterioration in FFPE sections. Top panels (a,b): CEA in normal gastric mucosa. Bottom panels (c,d): Cytokeratin in normal infantile thymus. Left (a,c): paraformaldehyde-fixed frozen sections. Right (b,d): FFPE sections. Antigenicities of CEA and cytokeratin detected by polyclonal antibodies are evidently weakened after the FFPE process. Arrows indicate Hassall’s corpuscles in the thymic medulla. The nuclei are counterstained with methyl green.
