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. 2021 Jun 15;10(6):1501. doi: 10.3390/cells10061501

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© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antigenic loss by fixation in organic solvent. Top panels (a,b): Immunostaining for platelet factor 4 in a smeared buffy coat of the peripheral blood. Bottom panels (c–f): Immunostaining for insulin (c,e) and somatostatin (d,f) in paraffin-embedded pancreas. Left (a,c,d): Fixation in acetone or ethanol. Right (b,e,f): Fixation in 4% paraformaldehyde or in formalin. Platelet factor 4 and insulin show false negative findings when fixed in the organic solvent. Somatostatin is resistant to ethanol fixation to be clearly immunolocalized. The nuclei are counterstained with methyl green.