Figure 8.

Demonstration of CD15 and EBER in Hodgkin’s lymphoma in a male patient in his 50s autopsied by Dr. Thomas Hodgkin himself 170 years ago. Top panels: H&E. Only one unstained slide of the liver available was stained with H&E. After photographing, the section was transferred to two silane-coated glass slides (a). Infiltration of Reed-Sternberg cells or Hodgkin’s cells is microscopically recognized (b). Bottom panels: Immunostaining for CD15 (c) and in situ hybridization for EBER (d) gave positive signals on the plasma membrane and in the nucleus, respectively, as indicated by yellow and red arrows.