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Demonstration of HCV antigens in an archival liver specimen of chronic hepatitis after HIER. (a): H&E, (b): monoclonal antibody against the NS3 region of HCV and (c): monoclonal antibody against the E1 region. Dot-like immunoreactivity of HCV antigens is seen in the cytoplasm of hepatocytes. The specimen was kept in formalin for 40 years.
