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. 2021 Jun 15;10(6):1501. doi: 10.3390/cells10061501

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© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antigenic loss of surface markers by endogenous peroxidase blockage in fresh-frozen sections. Aspirated bone marrow clot of essential thrombocythemia. Immunostaining for glycoprotein IIb/IIIa (CD41/CD61), a megakaryocytic marker. (a): Without endogenous peroxidase blockage and (b): with endogenous peroxidase blockage, inset: H&E. Endogenous peroxidase blockage in 0.3% hydrogen peroxide in methanol significantly hampers the immunoreactivity. The nuclei are counterstained with methyl green.