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Deterioration of antigenicity by protease pretreatment. Top panels (a,b): substance P in the myenteric plexus of the small bowel. Bottom panels (c,d): IgD in the mantle zone of the pharyngeal tonsil. Left (a,c): without treatment and Right (b,d): after trypsin digestion for 15 min. Immunoreactivities of substance P and IgD are lost or markedly decreased by the enzymatic pretreatment.
