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Application of HIER (II). Top panels (a,b): Vimentin in lung metastasis of anaplastic thyroid carcinoma (inset: H&E). Bottom panels (c,d): Parathyroid hormone in normal parathyroid gland. Left (a,c): Without HIER treatment and Right (b,d): after HIER with autoclaving at 121 °C for 10 min in 10 mM citrate buffer, pH 6. Immunoreactivities of vimentin and parathyroid hormone are significantly retrieved by the hydrated heating pretreatment.
