Figure 38.

Weakening of Ki-67 immunoreactivity by overnight antibody incubation after HIER with pressure pan heating at 121 °C for 10 min in 10 mM citrate buffer, pH 6.0. (a): One-hour antibody incubation and (b): overnight incubation. Ki-67 labeling in the germinal center lymphocytes of the pharyngeal tonsil, clearly seen in the 1-h antibody incubation, is significantly weakened after overnight incubation.