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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Antigenic loss by HIER in 10 mM citrate buffer, pH 6.0. BM-1 (a myeloid marker) in FFPE bone marrow aspiration. (a): Without HIER and (b): after HIER with pressure pan heating at 121 °C for 10 min. BM-1 immunoreactivity is quite susceptible to the heating pretreatment.