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. 2021 Jun 15;10(6):1501. doi: 10.3390/cells10061501

Figure 40.

Figure 40

Antigenic loss by HIER in 10 mM citrate buffer, pH 6.0. BM-1 (a myeloid marker) in FFPE bone marrow aspiration. (a): Without HIER and (b): after HIER with pressure pan heating at 121 °C for 10 min. BM-1 immunoreactivity is quite susceptible to the heating pretreatment.