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. 2021 Jun 15;10(6):1501. doi: 10.3390/cells10061501

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© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cross-reactive immunostaining of Mycobacterium leprae in the liver with a rabbit anti-HBs antiserum, immunized with complete adjuvant containing the extract of M. tuberculosis. (a): H&E, (b): anti-HBs rabbit antiserum, (c): anti-HBs monoclonal antibody. Leproma is seen mainly in the portal area. The antiserum visualizes M. leprae in the cytoplasm of macrophages richly distributed both in the portal triad (leproma cells) and in the sinusoid (Kupffer cells). The monoclonal antibody detects HBs antigen-positive hepatocytes (arrows) without background cross-reactivity.