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Re-staining of a cytology specimen with Chlamydia trachomatis infection. (a): Papanicolaou and (b): immunostaining with a mouse monoclonal antibody B104.1 after removal of the cover glass and decoloration. A cytoplasmic nevular inclusion body in a cervical columnar cell is clearly immunoreactive for the C. trachomatis antigen, confirming the cytodiagnosis of chlamydiasis. The nuclei in panel (b) are counterstained with methyl green.
