Figure 59.

Pre-embedding immunoelectron microscopy for SFTS virus using a TACAS glass slide. (a): Light microscopic chromogenic immunostaining and (b): immunoreactive round-shaped viral particles, around 100 nm in size, at the ultrastructural level. The FFPE splenic red pulp from an autopsy case was immunostained with a monoclonal antibody to SFTS virus after HIER. For pre-embedding immunoelectron microscopy, the immunostained section was peeled off for transfer to an Epon-embedded block by the inverted beam capsule method. The miraculous TACAS glass slide prevents detachment of sections during immunostaining and allows cell transfer after immunostaining. Bar indicates 500 nm.