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Intestinal spirochetosis caused by colonization of Brachyspira aalborgi. (a): H&E, (b–d): immunostaining using Treponema pallidum antiserum (b), BCG antiserum, (c) and E. coli antiserum (d). Long and basophilic spiral bacteria closely attach onto the apical surface of the colonic columnar cells (a). They are clearly immunostained with antisera to T. pallidum, BCG, and E. coli (b–d).
