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Bactericidal effect of (a) estafiatin and (b) eupatilin against Helicobacter pylori. Bacterial cultures were exposed to different concentrations (15.6 to 125 μg/mL) of (a) estafiatin or (b) eupatilin. Bacterial viability was assessed by the plate colony count technique at different times of incubation. All the experiments were carried out in triplicate and repeated at least three times. Clarithromycin (0.05 μg/mL) and metronidazole (300 μg/mL) were used as positive controls.
