Fig. 5. Role of TET enzymes in the rejuvenation event.
(A) Application of rDNAm clocks to TET triple KO (TET KO) and wild-type (WT) E6.5 epiblast samples (dataset 17). (B) Application of our recently developed single-cell clock [scAge; (49)] to TET KO and WT ESCs at days 2 and 5 of differentiation (dataset 18). Specific lineages and stages (Mapped lineage and Mapped E-day) were assigned by mapping the RNA expression profiles of the in vitro cells to an in vivo gastrulation atlas. Two-sided t tests were calculated (ns, P > 0.05; *, 1 × 10−2 < P ≤ 5 × 10−2; **, 1 × 10−3 < P ≤ 1 × 10−2; ***, 1 × 10−4 < P ≤ 1 × 10−3; ****P ≤ 1 × 10−4).