Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Bap1 | GenBank | MGI:1206586 | |
| Gene (Mus musculus) | Asxl1 | GenBank | MGI:2684063 | |
| Gene (Mus musculus) | Asxl2 | GenBank | MGI:1922552 | |
| Gene (Homo sapiens) | BAP1 | GenBank | HGNC:950 | |
| Cell line (Mus musculus) | Mouse trophoblast stem cell line | Prof. Rossant lab | TS-Rs26 | |
| Cell line (Homo sapiens) | Human trophoblast stem cell line | Prof. Arima lab | BTS5 | |
| Cell line (Homo sapiens) | HEK293T: Human embryonic kidney cells | ATCC CRL-3216 | HEK293T | |
| Cell line (Homo sapiens) | Choriocarcinoma cell line | ATCC HTB-36 | JEG-3 | |
| Cell line (Homo sapiens) | Choriocarcinoma cell line | ATCC HTB-144 | JAR | |
| Cell line (Homo sapiens) | Choriocarcinoma cell line | ATCC CCL-98 | BeWo | |
| Antibody | Anti-ACTIN (mouse monoclonal) | Abcam | ab6276 | WB (1:5000) |
| Antibody | Anti-ASXL1 (rabbit monoclonal) | Cell Signaling | #52519 | WB (1:1000), IF (1:100) |
| Antibody | Anti-ASXL1 (mouse monoclonal) | Abnova | H00171023-M05 | WB (1:1000), IP (1:100) |
| Antibody | Anti-ASXL2 (rabbit polyclonal) | Abcam | ab106540 | WB (1:1000), IF (1:100) |
| Antibody | Anti-ASXL2 (rabbit polyclonal) | Bethyl Laboratories | A302-037A | WB (1:1000), IP (1:250) |
| Antibody | Anti-BAP1 (rabbit monoclonal) | Cell Signaling | #13187 | IF (1:200), WB (1:1000), IHC (1:100), IP (1:250) |
| Antibody | Anti-E-Cadherin (CDH1) (mouse monoclonal) | BD Biosciences | 610181 | IF (1:200) |
| Antibody | Anti-CDX2 (mouse monoclonal) | Biogenex | MU392A-UC | WB (1:1000) |
| Antibody | Anti-ESRRB (mouse monoclonal) | R&D Systems | H6707 | WB (1:1000), IF (1:200) |
| Antibody | Anti-GATA3 (mouse monoclonal) | Invitrogen | MA5-15387 | WB (1:1000) |
| Antibody | Anti-HLAG (mouse monoclonal) | Bio-Rad | MCA2043 | WB (1:1000) |
| Antibody | Anti-ITGA5 (mouse monoclonal) | Santa Cruz Biotechnology | sc-376199 | IF (1:100) |
| Antibody | Anti-TUBULIN (rat monoclonal) | Abcam | ab6160 | WB (1:5000) |
| Antibody | Anti-Rabbit IgG (H + L)-HRP (goat polyclonal) | Bio-Rad | 170–6515 | WB (1:5000) |
| Antibody | Anti-Mouse IgG (H + L)-HRP (goat polyclonal) | Bio-Rad | 170–6516 | WB (1:5000) |
| Antibody | Anti-Rabbit IgG (H + L), Alexa Fluor 488 (goat polyclonal) | Thermo Fisher Scientific | A-11034 | IF (1:500) |
| Antibody | Anti-Mouse IgG (H + L), Alexa Fluor 568 (donkey polyclonal) | Thermo Fisher Scientific | A-10037 | IF (1:500) |
| Recombinant DNA reagent | Lenti dCas9-VP64-Blast (plasmid) |
Addgene | RRID:Addgene_61425 | Lentiviral plasmid to transfect HEK293T cells and package dCas9-VP64 viral particles |
| Recombinant DNA reagent | Lenti MS2-p65-HSF1-Hygro (plasmid) |
Addgene | RRID:Addgene_61426 | Lentiviral plasmid to transfect HEK293T cells and package MS2-p65-HSF1 viral particles |
| Recombinant DNA reagent | Lenti sgRNA (MS2)-puro (plasmid) | Addgene | RRID:Addgene_73795 | Lentiviral plasmid to transfect HEK293T cells and package sgRNA viral particles |
| Recombinant DNA reagent | psPAX2 (plasmid) | Addgene | RRID:Addgene_12260 | Lentiviral packaging plasmid |
| Recombinant DNA reagent | pMD2.G (plasmid) | Addgene | RRID:Addgene_12259 | VSV-G envelope expressing plasmid |
| Recombinant DNA reagent | pSpCas9(BB)−2A-GFP (PX458) (plasmid) | Addgene | RRID:Addgene_48138 | Plasmid to express Cas9 from Streptococcus pyogenes with 2A-EGFP, and cloning backbone for CRISPR-knockout sgRNA |
| Recombinant DNA reagent | pLV [Exp]-Puro- CMV > EGFP: mBap1[NM_027088.2] (lentiviral particles) |
VectorBuilder | Calves(VB210106-1179qkj)-C | Lentiviral particles to transduce and express mouse GFP-BAP1 |
| Recombinant DNA reagent | pLV [Exp]-Puro- CMV > EGFP: hBAP1[NM_004656.4] (lentiviral particles) |
VectorBuilder | Cat#LVS(VB210106-1177amh)-C | Lentiviral particles to transduce and express human GFP-BAP1 |
| Sequence-based reagent | Stealth small interfering RNA (siRNA) | Thermo Fisher Scientific | 1320003_MS23-25 | |
| Sequence-based reagent | Stealth small interfering RNA (siRNA) | Thermo Fisher Scientific | 1320003_MS36-38 | |
| Sequence-based reagent | Stealth RNAi siRNA Negative Control Kit | Thermo Fisher Scientific | 12935100 | |
| Peptide, recombinant protein | Alexa Fluor 568 Phalloidin | Thermo Fisher Scientific | A12380 | IF (1:500) |
| Chemical compound, drug | Lipofectamine RNAiMAX Transfection Reagent | Thermo Fisher Scientific | 13778 | |
| Chemical compound, drug | Lipofectamine 2000 Transfection Reagent | Thermo Fisher Scientific | 11668019 | |
| Commercial assay or kit | Vybrant cell adhesion assay kit | Thermo Fisher Scientific | V13181 |
|
| Software, algorithm | Fiji | Schindelin et al., 2012 | ||
| Software, algorithm | StarDist | Schmidt et al., 2018 | ||
| Software, algorithm | TrackMate | Tinevez et al., 2017 | ||
| Software, algorithm | MorphoLibJ | Legland et al., 2016 | ||
| Software, algorithm | R studio | R studio software | http://www.rstudio.com | |
| Software, algorithm | GraphPad Prism 8 | GraphPad software | http://www.graphpad.com |