Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain background (Mus musculus) |
PvalbCre (PV-IRES-Cre) | Jackson Laboratories | Stock# 008069 RRID:IMSR_JAX:008069 |
|
| Strain background (Mus musculus) |
Camk2αCre | Jackson Laboratories | Stock# 005359 RRID:IMSR_JAX:005359 |
|
| Strain background (Mus musculus) |
PvalbCre (PV-IRES-Cre) x Ai14 | Jackson Laboratories | Stock# 008069 Stock# 005359 |
Mouselines originally from Jackson Laboratories; in-house cross |
| Strain background (Mus musculus) |
Tg(GrpCre)KH288Gsat | Jackson Laboratories | MGI:4367023 RRID:MMRRC_037585-UCD |
|
| Strain background (Mus musculus) |
PvalbCre (PV-IRES-Cre) x Ai32 | Jackson Laboratories | Stock# 008069 Stock# 024109 |
In-house cross |
| Strain background (Mus musculus) |
Ai32 | Jackson Laboratories | Stock# 024109 RRID:IMSR_JAX:024109 |
|
| Strain background (Mus musculus) |
Ai14 | Jackson Laboratories | Stock# 007914 RRID:IMSR_JAX:007914 |
|
| Peptide, recombinant protein |
NeuroTrace 45/455 | Thermo Fisher Scientific | Cat#N21479 | 1:200 |
| Peptide, recombinant protein |
Streptavidin, Alexa Fluor 488 Conjugate |
Thermo Fisher Scientific | Cat#S11223 | 1:250 |
| Peptide, recombinant protein |
Streptavidin, Alexa Fluor 594 Conjugate |
Thermo Fisher Scientific | Cat#S11227 | 1:250 |
| Peptide, recombinant protein |
Streptavidin, Alexa Fluor 647 Conjugate |
Thermo Fisher Scientific | Cat#S21374 | 1:250 |
| Chemical compound, drug |
Picrotoxin | Sigma | CAS # 124-87-8, P1675-1Q | 50 µL solution made in house daily from powder |
| Chemical compound, drug |
Tetrodotoxin citrate | Alomone Labs | CAS # 18660-81-6 | 1 µL solution made in house daily |
| Chemical compound, drug |
4-Aminopyridine | Alomone Labs | CAS # 504-24-5 | 100 µL solution made in house daily |
| Antibody | Anti-GFP (Chicken polyclonal) | Abcam | ab13970 RRID:AB_300798 |
1:2000 |
| Antibody | Anti-mCherry (Rabbit polyclonal) | Abcam | ab167453 RRID:AB_2571870 |
1:2000 |
| Antibody | Anti-Chicken (Donkey polyclonal), biotin conjugated |
Jackson Immuno Research | RRID:AB_2313596 | 1:300 |
| Antibody | Anti-Rabbit (Donkey polyclonal), biotin conjugated |
Jackson Immuno Research | RRID:AB_2340593 | 1:300 |
| Software, algorithm | Python 3.7.6 | Python | RRID:SCR_008394 | https://www.python.org/ |
| Software, algorithm | MATLAB R2020a | MATLAB | RRID:SCR_001622 |