Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (H. sapiens) | CBX5 | GenBank | ||
| Cell line (H. sapiens) | U20S | ATCC | ATCC HTB-96 | RRID:CVCL_0042 |
| Transfected construct (H. sapiens) | 3' HP1α-AID- sfGFP 2A PuroR | Addgene | 127906 | RRID:Addgene_127906 |
| Transfected construct (H. sapiens) | Guide RNA/Cas9 plasmid pX330 human 3' HP1α gRNA | Addgene | 127906 | RRID:Addgene_127906 |
| Sequence-based reagent | Guide RNA sequence, 5’- acagcaaagagctaaaggag −3' | This paper | ||
| Transfected construct (H. sapiens) | HP1α-mCherry | This paper, in pHR vector | HP1α PCR from Addgene_17652 | |
| Transfected construct (H. sapiens) | HP1αI165E- mCherry | This paper, in pHR vector | Point mutant made with quickchange | |
| Antibody | Anti-HP1 alpha (rabbit monoclonal) | Abcam | ab109028 | (1:250) RRID:AB_10858495 |
| Antibody | anti-H3K9me2/3 (mouse monoclonal) | Cell Signaling | 5327 | (1:100) RRID:AB_10695295 |
| Antibody | anti-Lamin B1 (rabbit polyclonal) | Abcam | ab16048 | (1:500) RRID:AB_443298 |
| Antibody | anti-Lamin A/C (mouse monoclonal) | Active Motif | 39287 | (1:1000) RRID:AB_2793218 |
| Chemical compound, drug | Auxin (NaIAA) | Sigma | I5148 | 1 mM |
| Chemical compound, drug | Methylstat | Sigma | SML0343-5MG | 1 μM |
| Software, algorithm | Kappa, nuclear curvature | FIJI Schindelin et al., 2012 |