Fig. 6.

Effects of cellular cooperation and plasma on the inhibitory effects of recombinant human ZPI (250 nM) on isolated lipopolysaccharide (LPS)-stimulated monocytes. rhZPI was added 30 min before the addition of 1 ng/mL of LPS. ( A ) Effects of ZPI on peripheral blood mononuclear cells. ( B ) Variations of IL-6 production by LPS-activated monocytes in the presence of ZPI (250 nM) and increasing concentrations of normal plasma. ( C ) Variations of IL-6 production by LPS activated monocytes in the presence of ZPI (250 nM) and increasing concentrations of plasmas deficient in coagulation factors. Results are expressed as percentages of cytokines induced by LPS which were considered arbitrarily as 100%. Results (mean ± 1 SEM) are the average of 3 independent experiments in triplicate. ^* p  < 0.05, ^*** p  < 0.001 as compared to the production of cytokines in the presence of LPS alone [Student’s t -test ( A, B ) or Dunnett’s multiple comparisons test following one-way ANOVA ( C )].