Table 1. Effects of purified protein Z (PZ) on the inhibitory effects of recombinant human ZPI (rhZPI) on the secretion of IL-6 and TNF-α in LPS-stimulated whole blood.

Conc. (nM)	IL-6 inhibition (%)				TNF-α inhibition (%)
	PZ	ZPI	PZ and ZPI	p (ZPI vs. PZ and ZPI)	PZ	ZPI	PZ and ZPI	p (ZPI vs. PZ and ZPI)
62.5	5.7 ± 0.9	0.5 ± 0.9	19.9 ± 3.9 a	0.001	0.23 ± 9	1.2 ± 5	30.5 ± 2.3 a	0.006
125	17.4 ± 14.1	27.6 ± 3.9 b	35.1 ± 2.2	0.24	11.6 ± 4.5	33.7 ± 4.5 c	50.2 ± 9.7	0.185
250	21.8 ± 6	65.8 ± 3 d	71.6 ± 1.9	0.437	3.2 ± 7.8	75.1 ± 3.2 d	82.2 ± 6.8	0.786

Abbreviations: IL-6, interleukin-6; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; ZPI, Z-dependent plasma protease inhibitor.

Note: rhZPI and PZ, at equimolecular concentrations (Conc.), were added 30 minutes before the addition of LPS (10 ng/mL). Results are expressed as percentages of inhibition of IL-6 or TNF-α production. Results (mean ± 1 SEM) are the average of three independent experiments in triplicate.

^a p  < 0.01 as compared with the effect of ZPI alone.

^b p  < 0.05 (as compared with the production of cytokines in the absence of ZPI).

^c p  < 0.01 (as compared with the production of cytokines in the absence of ZPI).

^d p  < 0.001 (as compared with the production of cytokines in the absence of ZPI).