Fig. 4.

Summary of Cellular Immune Response

Cells were stimulated with a S1 peptides pool of peptides and incubated at 37⁰C for 24–48 h. Cells stimulated with CD3–2 mAb served as a positive control. IFN-γ (left) or IL-4 (right) were detected using an ELISpot assay. The mean of spot-forming units (SFU) counted in peptide pool stimulation triplicate was calculated and normalized by subtracting the mean of the negative control replicates (control media). Results were expressed as SFU per million PBMC. Bars indicate the mean values and error bars indicate standard deviations.