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. 2021 May 28;297(1):100843. doi: 10.1016/j.jbc.2021.100843

Figure 5.

Figure 5

PTRPLS G393E and PTRPLS-like Q457R mutants significantly destabilized B3GLCT. Thermo shift assays with SYPRO Orange were employed to analyze the protein stabilities by measuring the melting temperatures of the proteins. Melting temperatures of PTRPLS mutants (black) and PTRPLS-like mutants (blue) were compared with the melting temperature of the WT (Fig. S5). Statistical analysis was performed with one-way ANOVA analysis in Prism 7. Error bars, standard deviation, n = 6 from two batches of purified enzymes with three replicates for each batch. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, not significant.