Table 2.
Kinetic parameter | WT | Q457R | T179S | V245M | R337H |
---|---|---|---|---|---|
Vmax (nmol ⋅ min−1 ⋅ mg−1) | 43.21 | 23.56 | 32.44 | 24.63 | 27.73 |
KM (μM) | 25.47 | 17.39 | 20.94 | 18.74 | 21.89 |
kcat (min−1) | 2.42 | 1.32 | 1.82 | 1.38 | 1.55 |
Kinetic parameter | WT | Q457R | T179S | V245M | R337H |
---|---|---|---|---|---|
Vmax (nmol ⋅ min−1 ⋅ mg−1) | 43.21 | 23.56 | 32.44 | 24.63 | 27.73 |
KM (μM) | 25.47 | 17.39 | 20.94 | 18.74 | 21.89 |
kcat (min−1) | 2.42 | 1.32 | 1.82 | 1.38 | 1.55 |