Reagent | Final concentration | Amount |
---|---|---|
BSA | 1 mg/mL | 50 mg |
Goat serum | 3% | 1.5 mL |
Triton X100 | 0.1% | 50 μL |
EDTA (500 mM) | 1 mM | 100 μL |
1×PBS | 48 mL | |
Total | n/a | 50 mL |
Reagent | Final concentration | Amount |
---|---|---|
BSA | 1 mg/mL | 50 mg |
Goat serum | 3% | 1.5 mL |
Triton X100 | 0.1% | 50 μL |
EDTA (500 mM) | 1 mM | 100 μL |
1×PBS | 48 mL | |
Total | n/a | 50 mL |