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. 2021 Jun 19;2(3):100620. doi: 10.1016/j.xpro.2021.100620

Hybridization solution

Reagent Final concentration Amount
Formamide 70% 1.4 mL
Blocking reagent (10%) 0.5% 100 μL
Tris-HCl pH 7.2 (1 M) 10 mM 20 μL
EDTA (500 mM) 1 mM 100 μL
Probe (25 μM) 125 nM 10 μL
ddH2O 0.37 mL
Total n/a 2 mL