REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa Fluor® 647 anti-mouse IgD (clone 11-26c.2a)	BioLegend	Cat# 405708; RRID:AB_893528
Ki-67 Monoclonal Antibody, FITC, eBioscience™ (Clone SolA15)	Thermo Fisher Scientific	Cat# 11-5698-82; RRID:AB_11151330
CD21/CD35 Rat anti-Mouse, Biotin, eBioscience™ (Clone: 8D9)	Thermo Fisher Scientific	Cat# 15239489
Hamster Anti-CD3e Monoclonal Antibody, Unconjugated (Clone 500A2)	BD Biosciences	Cat# 553238; RRID:AB_394727
Goat anti-Hamster IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568	Thermo Fisher Scientific	Cat# A-21112; RRID:AB_2535761
SAv-Brilliant Violet 421™	BioLegend	Cat# 405225
Biological samples
Mouse C57BL/6Babr (spleen tissue)	Babraham Institute	C57BL/6Babr
Chemicals, peptides, and recombinant proteins
Triton™ X-100	Sigma-Aldrich	Cat# T8787
Tween® 20	Sigma-Aldrich	Cat# P1379
OCT Compound	Agar Scientific	AGR1180
Hydromount mounting medium	National Diagnostics	Cat# HS-106
Normal Goat Serum	Sigma-Aldrich	Cat# 566380
Bovine Serum Albumin	Jackson ImmunoResearch	Cat# 001-000-161
L-Lysine, Hydrochloride	Merck Life Sciences	4400-100GM
Sodium phosphate monobasic	Sigma-Aldrich	Cat# S0751
Sodium phosphate dibasic	Sigma-Aldrich	Cat# S3264
Sodium periodate	Sigma-Aldrich	Cat# S1878
Paraformaldehyde (PFA)	Sigma-Aldrich	Cat# P6148
Streptavidin/Biotin Blocking Kit	Vector Laboratories	Cat# SP-2002
Software and algorithms
ImageJ	Schindelin et al., 2012	https://imagej.nih.gov/ij/
ZEN black edition software	Zeiss	https://www.zeiss.com
Other
Superfrost Plus slides	VWR	Cat# 631-0108
ImmEdge™ Hydrophobic Barrier PAP Pen	2B Scientific Ltd	Cat# H-4000
MX35 Premier Disposable Low-Profile Microtome Blades	Fisher Scientific UK Ltd	Cat# 3052835
Cryostat, CM1850	Leica	N/A
Zeiss LSM780 Confocal	Zeiss	N/A