Fig. 4.

Ethanol induces a modest and reversible inhibition of pharyngeal pumping in bus-17 mutants.

bus-17 deficient-mutants feeding in presence of a chronic incubation with DMSO (0.05–1 %) (A), as well as ethanol (1%) (B), present a significant reduction over time (showing with a light grey shadow block on the graph) in pumping rate compared to animals in untreated conditions (**** p ≤ 0.0001; n = 5). Acetone does not affect the pharyngeal pumping over time (ns, P > 0.05. n=6) (C). Data represent the mean ± s.e.m of pharyngeal pumping rate. The means were calculated from data collected from repeats of the same experiment conducted on different days. Statistical analysis was performed using two-way ANOVA (Bonferroni multiple comparisons test).