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. 2021 May 20;297(1):100775. doi: 10.1016/j.jbc.2021.100775

Table 1.

Effect of pyruvate on purified LDH enzyme kinetics

LDH enzymes Vmaz (ΔAbs/s∗10−3/μg protein) Vpeak (ΔAbs/s∗10−3/μg protein) Cmax (mM) Km (mM) Ki (mM)
LDH bovine heart 3.23 ± 0.10 2.08 0.48 0.13 ± 0.010 1.75 ± 0.11
rLDHA 1.00 ± 0.04 0.75 0.90 0.15 ± 0.01 5.33 ± 0.49
rLDHB 1.00 ± 0.05 0.75 0.48 0.08 ± 0.01 2.88 ± 0.40

Abbreviation: LDH, lactate dehydrogenase.

Summary of maximal initial velocities from activation curve fits (Vmax), peak initial velocities of the biphasic curve fits (Vpeak), pyruvate concentrations at peak velocities of the biphasic curve fits (Cmax), and apparent Km and Ki values of commercially available LDH enzymes from bovine heart, human recombinant LDHA and recombinant LDHB. All experiments were done in triplicate (technical replicates); n = 3, independent experiments (biological replicates); mean values with standard errors.